Food traceability and authentication - DNA
10 important questions on Food traceability and authentication - DNA
Why can you use DNA for species traceability and food authentication?
What approche is used to link DNA to species authentication?
Why can it be hard to extract DNA from food?
- Very lipophilic matrices (fats and oils) in which very little amount of DNA is present
- Very complex matrices with surfactants and emulsifier which hamper extraction
- Presence of specific interferents able to inhibit PCR, mostly polyphenols
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If you have a negative result in DNA analysis is this really a negative result?
What is DNA barcoding?
The gene order is put together like a barcode
What are the advantages of DNA barcoding?
- Conventional and inexpensive protocals for DNA extraction, amplification and sequencing. Thus reference library increases, enabling the rapid identification with specific short-DNA sequences.
- New Sequencing techniques such as prysoquencing enable rapid and representative analysis of mixed samples.
Can you also use DNA barcoding for plans?
Is a negative result prove that no other DNA is present?
A negative results in to sufficient to tell that a DNA is absent, because usually the limit of detection is not known, so they should be uses only as a positive confirmation, since not detected DNA can only be, actually, not detectable, which does not mean absent.
What is the advantage of using DNA to authenticate food stuffs?
- Strong rationale providing clear cut results (if the method applied is well developed in order to avoid ambiguities and DNA is extractable and can be amplified)
- Can be applied to almost every foodstuff, since DNA is everywhere
What is the disadvantage of using DNA to authenticate food stuffs?
- Authentication only of species/varieties/breeds, so whatever is genetically different. Can not authenticate place of production, technology, processing
- Accurate quantitation might be difficult, the technique is better suited to present/not present answers
- In heavily processed food DNA can be difficult to amplify, giving false negative results
- Mixure of many different species might complicate the analysis
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