Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Capillary electrophoresis of carbohydrates
5 important questions on Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Capillary electrophoresis of carbohydrates
What is a capillary electrophoresis?
What is the setup of capilarry electrophoresis?
The cations (+) will move to the cathode (-)
The anions (-) will move to the anode (+)
The neutral molecules will not wand to move at all
What are the properties that affect mobility in capillary electrophoresis?
- Voltage
- Size and charge of the molecule
- Viscosity of the buffer
- Electro Osmotic Flow (EOF)
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What is Electro Osmotic Flow (EOF)?
What are the detectors that can be used for capillary electrophoresis?
- UV detector
- DAD detector (diode array detector)
- LIF (laser induced fluorescence detector)
- Mass spectrometry
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