Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Capillary electrophoresis of carbohydrates

5 important questions on Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Capillary electrophoresis of carbohydrates

What is a capillary electrophoresis?

This is a setup that can separate molecules based on there charge by putting a electric field on them. By this you can ditermone the size, shpaen and charge of the molecules.

What is the setup of capilarry electrophoresis?

There is a catode and an anode.
The cations (+) will move to the cathode (-)
The anions (-) will move to the anode (+)
The neutral molecules will not wand to move at all

What are the properties that affect mobility in capillary electrophoresis?

  • Voltage
  • Size and charge of the molecule
  • Viscosity of the buffer
  • Electro Osmotic Flow (EOF)
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What is Electro Osmotic Flow (EOF)?

You have negatively charged silica on the tube. The cations (+) will accumulate near the surface. They will also like to move cathode (-) and this will create a flow called EOF.

What are the detectors that can be used for capillary electrophoresis?

  • UV detector
  • DAD detector (diode array detector)
  • LIF (laser induced fluorescence detector)
  • Mass spectrometry

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