Chromatography - Gas Chromatography (GC)
4 important questions on Chromatography - Gas Chromatography (GC)
What are the 2 different elution modes?
Gradient: temperature is gradually increased during the run. If you have no idea what you are going to find.
What is the most common GC detector?
- FID (flame ionization detection): The signal is generated by electrons created when combusting the eluting analytes. It is general and very sensitive, but it does not give any clue on the identification of unknown compounds
- MS (mass spectrometry): The detector is a mass spectrometer. It is general, fairly sensitive and also allows to get information useful for the identification of the unknown peaks in the chromatograms.
How can you analyse AA with GC?
So see if it is a D or a L AA.
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How can you analyse Fatty acid methyl esters (FAME) with a GC?
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