Analysis of Carbohydrates - Examples of carbohydrate analysis
3 important questions on Analysis of Carbohydrates - Examples of carbohydrate analysis
How do you do a dietary fiber analysis?
How does the AOAC method for GOS analysis work?
- GOS is extracted in hot water
- GOS is hydrolyzed with beta-galactosidase
- Sample is analysed with HPAEC
- Also blank sample without enzyme treatment is analysed with HPAEC (in order to compensate for the natural present lactose)
What are the advantages and limitations of the AOAC GOS method?
- The only validated & official method
- Easy protocol
- Possible for most laboratories
Limitations
- No information on the individual compounds of GOS
- Assumed knowledge on GOS DP
- Potential error in products containing high levels of free galactose or lactose
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