Analytic separation techniques for carbohydrates - Chromatography of carbohydrates - Hydrophilic interaction chromatography (HILIC)
4 important questions on Analytic separation techniques for carbohydrates - Chromatography of carbohydrates - Hydrophilic interaction chromatography (HILIC)
For what his HILIC used in carbohydrate detection?
- Retention based on size and charge density
- Separation strongly depends on structure of oligosaccharides
- The column binds the carbohydrates and when the water content of the eluent is increased they will come off.
- Elution at low pH will enable separation of both neutral and acidic oligosaccharides.
What is the detector used for HILIC?
What is the disadvantage of HILIC-ELSD that you can see on this picture?
For the ones under that allmost linear.
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On what does separation of HILIC depend?
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
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- 100% sure, 100% understanding
