Analytic separation techniques for carbohydrates - Chromatography of carbohydrates - High performance anioni-exchange chromatography (HPAEC)
4 important questions on Analytic separation techniques for carbohydrates - Chromatography of carbohydrates - High performance anioni-exchange chromatography (HPAEC)
For what can HPAEC in carbohydrates be used?
How does HPAEC work?
The stationary phase is positively charged and the negatively charged carbohydrates will get attached to it. You will give a negative charge to the molecules by putting them in a high pH.
The attachment is based on the PKs value of the OS and it size and linkages present.
What is the detector used for HPAEC?
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What is the conclusion about HPAEC?
- High resolution for oligomers
- Quite useful and flexible in oligosaccharide separation
- Carbohydrate structure not always predictable form elution behavior.
- Alkali labile groups lost (methyl esters, acetyl groups)
- Elution with salts requires desalting prior to MS-detection
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