Mass spectrometry - Using mass spectrometers as detectors for chromatography: hyphenation
5 important questions on Mass spectrometry - Using mass spectrometers as detectors for chromatography: hyphenation
How can you combine GC with mass spectrometry?
How can you combine LC with mass spectrometry?
Reversed Phase is ok (organic + water + acid modifier is perfect for ESI)
Ion Exchange is not ok (too many salts in the eluent)
Normal Phase is also not ok (eluent not suitable for ionization)
HILIC is ok (same solvent system as RP)
What is a full scan (total ion current TIC)?
Every molecule will have a full MS spectrum associated.
This is a untargeted analysis
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What is single ion monitoring (SIM)?
More selective and sensitive.
No MS spectrum for the compounds: no confirmation
This is a targeted analysis
What is selected reaction monitoring (SRM)?
Targeted Analysis
- A specific ion is selected in the first quadrupole, fragmented, and only a specific ion is selected by the third quadrupole to reach the detector.
- Conceptually is like doing a Single Ion Monitoring in both quadrupoles.
- Loss of all the data not acquired
- The results is the detection of specific fragments which originates only from specific preselected compounds.
- The advantage is the extreme sensitivity and specificity in complex mixtures.
- If more than one ion is selected, the acquisition mode is called Multiple Reaction Monitoring (MRM)
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