Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis

5 important questions on Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis

What happens if your hydrolysis conditions are to mild?

  • Not a representative composition
  • Insoluble fibres, uronic acids and cellulose difficult to hydryose complete
  • No generic method for both soluble and insoluble polysaccharides.

What happens if your hydrolysis conditions are to harsh?

Sensitive sugars are lost more readily

How do you hydrolyse fibrous materials?

  • 12 M H2SO4 for 1 hour at 30 degrees
  • 1M H2SO4, 3H at 100 degrees.
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How do you hydrolyse uronic acid containing materials (pectin)?

2M HCl in methanol, 16h at 80 degrees

How can you analyse sugar composition with GC?

By first a reduction step (bound O become a OH) and then do a acetylation step so the sugar becomes volatile.

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