Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis
5 important questions on Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis
What happens if your hydrolysis conditions are to mild?
- Not a representative composition
- Insoluble fibres, uronic acids and cellulose difficult to hydryose complete
- No generic method for both soluble and insoluble polysaccharides.
What happens if your hydrolysis conditions are to harsh?
How do you hydrolyse fibrous materials?
- 12 M H2SO4 for 1 hour at 30 degrees
- 1M H2SO4, 3H at 100 degrees.
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How do you hydrolyse uronic acid containing materials (pectin)?
How can you analyse sugar composition with GC?
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