Bioassays - Introduction and general principle - An organism
4 important questions on Bioassays - Introduction and general principle - An organism
On what are in vivo models based?
What are the positive things of a organismal bioassay?
- They react on a white range of chemicals
- Reflects the actual risks that chemicals in food may pose to organisms.
- Some compounds are activated trough the metabolic pathways of an organism in order to become toxic.
What is the mouse bioassay (MBA)?
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What are the drawbacks of the in vivo BMA?
- unethical: the sacrifice of a large number of animals is involved
- a colony of mice between 19 g and 22 g in weight must be maintained
- detection limit of the assay is strain-dependent - death time versus toxin level is non-linear
- very labour-intensive to determine accurately the death time
- sensitive to confounding factors many false positives and negatives
- not sufficiently sensitive in detecting the toxin for the protection of human health
In spite of these difficulties, MBA still is the official method in many countries
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