Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Chromatography of carbohydrates
4 important questions on Analysis of Carbohydrates - Analytic separation techniques for carbohydrates - Chromatography of carbohydrates
Which eluent/gradient do you use for the different carbohydrates?
- Water elution + post column alkali addition
monomers - NaOH elution
Monomers and dimers - NaOH gradient
Mono-, di-, tri-, tetramer - NaAc gradient in e.g. 100mM NaOH
Higher neutral oligomers; acid oligomers
For what is reversed phase chromatography used?
- good separation of neutral and oligosaccharides, no retention of acidic oligosaccharides
- α/β can not be separation
- Separation based on polarity, polair groups will dilute wile apolar groups will stick to the column.
What is porous graphitized carbon (PGC) liquid chromatography?
- Retention mainly based on planarity (vlakheid) of oligosaccharides
- Separation strongly depends on structure of oligosaccharides
- Acidic oligosaccharide retention mainly based on net charge
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Can you use the GC to determine the structure and composition of pectin?
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