Analysis of Carbohydrates - Classical carbohydrate methods
4 important questions on Analysis of Carbohydrates - Classical carbohydrate methods
How does a refractometer work?
It measures the refraction index of a sample.
VERY fast method!
- Influenced by presence of other compounds for instance organic acids
- Value influenced by composition of sample.
- not very specific
How does a colour assay work?
However some sugar molecules might have more affinity to bind with the phenolic compound which introduces a error.
So it can be used as a indication but is not very specific.
How does the Nierle Tegge assay go?
The Cu2+ is present in a EDTA complex. When the reaction occurs you will get a free EDTA complex.
The amount of free EDTA can be quantified with a titration with Cu2+.
Unfortunately not all sugars react the same and therefore the results are a rough estimation.
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What is the dextrose equivalent (DE)?
DE does not necessarily provide information about the distributor of maltodextrin present.
Easy to standardize by adding glucose
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