Analysis of Carbohydrates - Enzymatic methods for carbohydrate analysis - Starch analysis by enzyme kits
3 important questions on Analysis of Carbohydrates - Enzymatic methods for carbohydrate analysis - Starch analysis by enzyme kits
How do you determine the amount of digestible starch in your sample?
- Remove free glucose and maltodextrines by waching with 70% ethanol in water.
- Gelatinize starch
- Incubate starch sample with alfa-amylase and amyloglucosidase in buffer
- Analyse sample.
How do you determine the amount of resistant starch in your sample?
- Solublelize RS in KOH or DMSO
- Change solutiong to the right pH
- Add enzymes alfa amylase
- Oxidize glucose in order to quantify it with a colour assay.
- Does not work for RS4 (chemically modified starch)
How do you analyse RS4?
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