Analysis of Carbohydrates - Enzymatic methods for carbohydrate analysis - Starch analysis by enzyme kits

3 important questions on Analysis of Carbohydrates - Enzymatic methods for carbohydrate analysis - Starch analysis by enzyme kits

How do you determine the amount of digestible starch in your sample?

  • Remove free glucose and maltodextrines by waching with 70% ethanol in water.
  • Gelatinize starch
  • Incubate starch sample with alfa-amylase and amyloglucosidase in buffer 
  • Analyse sample.

How do you determine the amount of resistant starch in your sample?

  • Solublelize RS in KOH or DMSO
  • Change solutiong to the right pH
  • Add enzymes alfa amylase
  • Oxidize glucose in order to quantify it with a colour assay.
  • Does not work for RS4 (chemically modified starch)

How do you analyse RS4?

By acid hydrolysis followed by determination of glucose release by GC or HPAEC

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