Analysis of Carbohydrates - Sample preperation - Derivatisation
3 important questions on Analysis of Carbohydrates - Sample preperation - Derivatisation
What is the aim of derivatisation of carbohydrates?
The aim is to modify a carbohydrate into a product of similar chemical structure which is easier to separate or to detect.
How can you analyse glycosidic linkages between sugar molecules?
First you do methylation
Then you do hydrolyses, deuterized reduction and acetylation.
Then you do hydrolyses, deuterized reduction and acetylation.
Why are sugars labeld with APTS?
Done to give betters separation (CE) or more sensitive detection (spectrophotometer).
The reducing end of a molecule reacts with APTS
- To give (negative) charge to a sugar molecule
- Label at reducing end of the molecule
- To increase signal (fluorescent)
- To allow quantification on mole basis
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