Bioassays - Bioassays in food safety - Additives
4 important questions on Bioassays - Bioassays in food safety - Additives
What are the types of bioassays you need to perform on additives (E-numbers)?
- Require extensive safety testing using in vitro and in vivo bioassays before they can be added to food
- In vivo assays for defining target tissue and dose response curves for setting safety limits (NOAEL, BMDL for defining the ADI)
- In vitro bioassays to test for absence of genotoxicity
What is the Ames Test with Salmonella typhimurium?
What are the possible bioassays for allergenicity?
- Double-blind, placebo-controlled food challenges (Human vollenteers)
- Measure total (or specific) IgE levels with Elisa bioassay (human volunteers)
- Skin prick test (human volunteers)
- Ex vivo histamine release
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How does ex vivo histamine release go?
- Based on the release of histamine by mast cells of actively sensitized guinea pigs
- One s.c. injection of the allergen and human serum albumin (HSA) as reference (standard) allergen.
- Three weeks later mast cells are isolated, challenged in vitro with of the test allergen and HSA
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