Optical Sectioning Techniques - Image Deconvolution - Spinning Disk Mic

4 important questions on Optical Sectioning Techniques - Image Deconvolution - Spinning Disk Mic

Imaging speed in SDM...

Is limited by scan speec of laser of focus

A pattern of multiple... (SDM)

Focal points sweeps the sample, where a disk with holes that turns take the pinhole function.

A second disk...

With microlenses focuses the excitation beam on to th eholes in the Nipkow disk.
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Three SDM advantages

1. Each location is illuminated 1000 times per second
2. THe time during which a point of the sample is illuminated is longer compared to LSCM, so a lower excitation energy can be used --> less bleaching
3. Quantum efficiency up to 90%

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