Optical Sectioning Techniques - Image Deconvolution - Spinning Disk Mic
4 important questions on Optical Sectioning Techniques - Image Deconvolution - Spinning Disk Mic
Imaging speed in SDM...
A pattern of multiple... (SDM)
A second disk...
- Higher grades + faster learning
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- 100% sure, 100% understanding
Three SDM advantages
2. THe time during which a point of the sample is illuminated is longer compared to LSCM, so a lower excitation energy can be used --> less bleaching
3. Quantum efficiency up to 90%
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