Reporter genes - Getting the reporter gene in cells - Transient expression
5 important questions on Reporter genes - Getting the reporter gene in cells - Transient expression
How do you check for representative activity with stable transformation?
What is an advantage of transient expression over stable transformation?
Name 5 transient expression methods?
2. Particle bombardment
3. Micro-injection DNA
4. Transfection DNA (elektro, PEG, heatshock)
5, Viral infection
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Particle bombardment, how does it work?
Transfecting protoplast works as follows...
By chemical, heat, or electrical treatment these cells can uptake the DNA constructs.
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