Reporter genes - Getting the reporter gene in cells - Transient expression

5 important questions on Reporter genes - Getting the reporter gene in cells - Transient expression

How do you check for representative activity with stable transformation?

Screen multiple independent transformants to check for similar activities.

What is an advantage of transient expression over stable transformation?

Much more gene copies can be transferred at once.

Name 5 transient expression methods?

1. Agro-infiltration leaf
2. Particle bombardment
3. Micro-injection DNA
4. Transfection DNA (elektro, PEG, heatshock)
5, Viral infection
  • Higher grades + faster learning
  • Never study anything twice
  • 100% sure, 100% understanding
Discover Study Smart

Particle bombardment, how does it work?

Gold particles are coated with DNA that has the reporter construct. Plant tissue is then bombarded with these coated particles. Cells that survive the bombardment can then express the DNA that was introduced.

Transfecting protoplast works as follows...

Protoplasts are suspended in solution with reporter construct DNA.
By chemical, heat, or electrical treatment these cells can uptake the DNA constructs.

The question on the page originate from the summary of the following study material:

  • A unique study and practice tool
  • Never study anything twice again
  • Get the grades you hope for
  • 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
Trustpilot Logo