Light-activated experiments and super-resolution imaging - Super Resolution; beating the diffraction limit - PALM

3 important questions on Light-activated experiments and super-resolution imaging - Super Resolution; beating the diffraction limit - PALM

The principle of Photo Activated Localization Microscopy is...

Activating photoactivatable-GFP by a specific wavelength, after which the temporarily activated fluorophores can be images.

Three disadvantages of PALM are...

1. Bleaching
2. Too long for live-cell imaging
3. Hundreds of cycles needed to collect the final image

A disadvantage of photoactivatable fluorophores is that...

They bleach faster

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