Fluorescence in Biology - Ratiometric imaging
4 important questions on Fluorescence in Biology - Ratiometric imaging
Also FRET assays can benefit from ratiometric imaging because...
Name some advantages of ratio probes...
2. Fast (live-cell suitable)
3. Accurate spatiotemporal information
4. Independent of probe concentration, light intensity and photobleaching
Confocal microscopy compared to ratio-metric imaging can (2) ... But...
2. Create 3d projections
But; resolution limited by diffraction of light
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Ratiometric imaging has as an advantage compared to CM...
And is independent of probe concentration, light intensity and photobleaching.
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