Fluorescence in Biology - Ratiometric imaging

4 important questions on Fluorescence in Biology - Ratiometric imaging

Also FRET assays can benefit from ratiometric imaging because...

The signal-to-noise ratio is greatly improved

Name some advantages of ratio probes...

1. Easy implementable
2. Fast (live-cell suitable)
3. Accurate spatiotemporal information
4. Independent of probe concentration, light intensity and photobleaching

Confocal microscopy compared to ratio-metric imaging can (2) ... But...

1. Block out-of-focus light
2. Create 3d projections

But; resolution limited by diffraction of light
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Ratiometric imaging has as an advantage compared to CM...

Easy, fast qualitative measurements.
And is independent of probe concentration, light intensity and photobleaching.

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