Fluorescence microscopty - Fluorescence in General

3 important questions on Fluorescence microscopty - Fluorescence in General

What molecules often show fluorescence?

Aromatic molecules, because these are easier excitable

4 light sources for fluorscence are:

1. Halogen/Tungsten lamp; light intensity distributed over a continuous spectrum
2. Gas discharge lamp; Light spectrum with intense peaks
3. LED's; Narrow wavelength band
4. Lasers; High intensity in a single wavelength.

Why not starte directly into the excitation source?

Because no fliter (excitation filter) blocks 100% effectively.

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