Fluorescence microscopty - Optical Sectioning Techniques

4 important questions on Fluorescence microscopty - Optical Sectioning Techniques

With Image Deconvolution the sample...

Is moved through the focal plane step by step

Fluorophores in the out-of-focus plane (CLSM)...

Are still photobleached, because they do get excited by the used laser.

Total Internal Reflection Fluorescence Mic illuminates the sample...

Through the objective by a shallow angle light ray that reflects at the cover glass-water interface. At an angle of 62 degrees, light is fully reflected except fo a evanescent light field that penetrates about 100 nm into the water.
  • Higher grades + faster learning
  • Never study anything twice
  • 100% sure, 100% understanding
Discover Study Smart

This reflection of the light in TIRF results in...

That only the fluorophores near the interface are excited.

The question on the page originate from the summary of the following study material:

  • A unique study and practice tool
  • Never study anything twice again
  • Get the grades you hope for
  • 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
Trustpilot Logo