Reporter genes - Reporter proteins - Bioluminiscence

4 important questions on Reporter genes - Reporter proteins - Bioluminiscence

For what can luciferase be used in plants?

Promoter activity, because there is no LRE so it is a direct measure of promoter activity.

How can you test in vivo dynamic studies with LUC?

Fuse LUC to a signal peptide gene. If ATP is released, then also oxygen is released which reacts with luciferin and luciferase.

An example of a bioluminiscence reporter protein is...

Luciferase (enzyme)
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How can you check if a jump in bioluminiscence is due to (1) increased promoter activity or (2) temperature change?

Make luciferase constructs with different promoters.
1. If all give the same response, then it is due to temperature.
2. If there is a different response, then is is due to promoter effect.

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