Fluorescence in Biology - Fluorescence in general

4 important questions on Fluorescence in Biology - Fluorescence in general

Name 5 advantages of fluorescence as a method

1. Sensitive measuring
2. Specific measuring
3. Multiparameter measuring
4. Living cell imaging
5. Dynamics can be imaged

Name 5 disadvantages of fluorescence as a method

1. Labelling and introduction into cell time costly
2. Phototoxic effects
3. Sample thickness restricted
4. Fluorophore disturbance

With choosing a dye, many factors are involved such as... (7)

1. Absorption/emission spectra
2. Extinction coefficient
3. Quantum yield
4. Environment sensitivity
5. Method of labeling
6. Photobleaching
7. Size
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The quantum yield is dependent of... (4)

1. Temperature
2. Ionic strength
3. PH
4. Excitation light intensity

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