Resolution and contrast generation - Phase contrast microscopy

6 important questions on Resolution and contrast generation - Phase contrast microscopy

Illumination of PC microscopy happens with...

A hollow cone of light, which causes a 1/4 phase shift, while the objective lens causes an additional 1/4labda phase shift.

How is the light imaged tot the same location?

Scattered AND non-scattered light are imaged tot the same location, where destructive interference then darkens the image to create contrast.

Ways of work: brightfield vs darkfield

Brightfield --> small amplitude differences
Phase contrast --> destructive interference
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Limitations of phase contrast microscopy

Halo's around the objects

Phase contrast microscopy works best for...

Thin objects that cahnge the phase of light by 1/4 labda

Phase cotrast microscopy most used for...

Live cell imaging

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