Fluorescence in Biology - Fluorescent label

6 important questions on Fluorescence in Biology - Fluorescent label

The advantage of chemical dyes

1. High quantum yield

3 Disadvantages of chemical  dyes...

1. Labeling & purification
2. Environment sensitive
3. Sticking to intracellular components

2 Advantages of intrinsic fluorophores...

1. No labelling & purification
2. No extension of molecule of interest
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3 Disadvantages of intrinsic fluorophores...

1. Low quantum yield
2. Photobleaching sensitive
3. Selective (only those that have them can be studied)

4 Advantages of quantum dots

1. Photostable
2. Different beads excited at one wavelength
3. Sharp emission bands
4. High extinction coefficients

A disadvantage of quantum dots...

1. Size

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