Light-activated experiments and super-resolution imaging - Light-activated experiments
3 important questions on Light-activated experiments and super-resolution imaging - Light-activated experiments
If the timescale of diffusion is fast compared to the timescale of protein-target dissociation...
Then the fluorescence recovery is a direct measure of the bond.
But a disadvantage of uncaging is...
You need to get the caged molecules into the cell which is time-consuming.
Examples of caged molecules are...
ATP, Ca2+, cAMP (second messengers)
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