Optical Sectioning Techniques - Confocal Laser Scanning Mic - Two-photon Mic
3 important questions on Optical Sectioning Techniques - Confocal Laser Scanning Mic - Two-photon Mic
TP Mic is a laser scanning technique that...
Can excite and detect fluorescence deep inside opaque samples, multiple cell layers deep.
3 TP Mic advantages
1. Infrared light penetrates deeper into tissues compared to visible light.
2. No excitation and out-of-focus photobleaching
3. No need for pinholes to block out-of-focus light.
2. No excitation and out-of-focus photobleaching
3. No need for pinholes to block out-of-focus light.
2 TP Mic disadvantages
1. More complex pulsed layers are needed tat deliver a high instantaneous intensity
2. More photobleaching due to high intensity lasers
2. More photobleaching due to high intensity lasers
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