Protein denaturation, protein stability - Measuring protein unfolding - Differential scanning calorimetry
4 important questions on Protein denaturation, protein stability - Measuring protein unfolding - Differential scanning calorimetry
What is the information you get from DSC (differential scanning calorimetry)?
- Heat capacity (Cp) of native and unfolded proteins
- Denaturation temperature
- Enthalpy of unfolding
How can you determine how much your sample is unfolded with DSC?
The more bonds that need to be broken the more energy you need. So the higher the peak the less denatured proteins your sample has.
How does the heterogeneity (different proteins, or isoforms influence the analysis of DSC results?
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For what is DSC used and explain how to read the results
The unfolding curve represents the probability density function of protein unfolding. This curve can be transformed to a cumulative distribution function, which shows the fraction of folded proteins as a function of temperature. This can be used to calculate free energy of unfolding (∆Gunf)
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