Phenolic Compounds - Isolation of phenolic compounds
15 important questions on Phenolic Compounds - Isolation of phenolic compounds
What is the pretreatment of a sample if you wand to isolate the phenolic compounds?
This is to extracht the fatty acids.
However not always good because some phenolics might be so apolair that they go into the hexaan. However often not the case.
How do you choose your solvent for phenolic compound extraction?
How can you change the polarity of the solvent?
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What is a quantative extraction?
How do you get a quantative extraction and how can you check it?
You can check if you have everything by the spectophotometer.
How do you measure with the spectophometer?
Or if you know which phenolic compounds you have you can check for there specific absorbance.
Why has the daizein a lower absorbtion spectrum then coumestrol?
Can proteins form give a false result in the photospectrum?
Only if you used a lot of water in your solvent.
With 100% ethanol this will not be the case. Then you only extract the small molecules.
When do you stop extracting with your solvent?
How do you ditermone how much solvent you are adding?
More solvent: less extraction steps and the other way around.
How does solid phase extraction go?
- You put your sample in a colomn and the apolair compounds(phenolics) will bind to the colomn. The polair compounds will not bind and go trough the colomn.
- Then you wash it with water in order to get the left over carbohydrates out.
- Then put in the eluting solvent to get the phenolic compounds out.
- Concentrate it by evaporating the eluent.
What is important in solid phase extraction of this compound?
How does partitioning or liquid-liquid extraction go?
So separation is based on difference in relative solubility.
How can you concentrate a sample by freeze-drying?
It will result in a fluffy homogenious sample.
How can you determine the amount of phenolics you have?
- By Absorbance at specific wave length (inaccurate)
- Colorimetic assac according to Folin-Ciocaltueu (incaccurate)
- HPLC analyses
- Calibration curve relating peak area to quantity of each compound
- Molar extinction coefficients
- Availability of standards poor: calculate as equivalents of related compound
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