Lipids - Analysis
8 important questions on Lipids - Analysis
How can you analyse fatty acid composition?
- Triacylglycerides are hydrolysed and methyl esterified
- FAMEs (fatty acid methyl esters) are applied to GC-FID
● peaks identification by comparing rt with rt of pure fatty acids
● Area of the peak: concentration of fatty acid
What is the relation between retention time and kind of fatty acid / FAME?
- The longer the FAME the longer the retention time
- The more saturated the FAME the longer the retention time
- Place double bond (W11<W7<W3<no W)
Can you distinguish between the cis and trans conformation in a GC-FID and the place where the double bond is?
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How can you calculate the degree of unsaturation and how is done?
- IV is grams of iodine reacted with 100 g of oil -
- Iodine reacts with the carbon-carbon double bonds -
- IV is a measure for the degree of unsaturation
Have saturated or ussaturated fat's a higher iodine value?
Which values would you use when calculating the IV for a lipid based on the fatty acid composition (analysed with GC): IV of fatty acid, IV of TAG, or IV of FAME?
What is the IV value of sunflower oil based on the facomposition: 11% 16:0, 6% 18:0, 29% 18:1 and 52% 18:2.
Becasue you are intrested in the oil you take the trigliceride values
How can you calculate the ECN (equivalent carbon number ) number and what does this number indicate?
The number indicates the order the triglycerides will appair on your HPLC diagram.
ECN= CN – 2n
- CN is the carbon number (without glycerol)
- n is the number of double bonds
More precisely: ECN = CN – d(o)*n(o) – d(l)*n(l) - d(ln)*n(ln)
- taking into account the origin of the double bond
- n(o) , n(l) and n(ln) are the numbers of double bonds attributed to oleic, linoleic and linolenic acids
- the coefficient do , dl and dln can be calculated by means of the reference triglycerides
Often used:
ECN = CN – 2.60n0 – 2.35nl – 2.17nln
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