Polysaccharides and health - Fibers - Determination of dietary fibre
6 important questions on Polysaccharides and health - Fibers - Determination of dietary fibre
What are the two main methods to analyse diatary fibers?
- Enzymatic/gravimetric method
- Uppsala method
What is the main difference between the enzymatic/gravimetirc method and the uppsala method?
How does the enzymatic/gravimetric method work?
A enzyme is added to remove the digestible starch.
The soluble non-starch polysccharides are removed with 80% ethanol.
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How does the uppsala method work?
Non-starch polysacharides are separated by 80% ethanol precipitation.
After drying the residue contains bout soluble and insoluble fibers.
You can split them with a filtration step.
Neutral sugars are determentin with the HPAEC.
Are all oligosacharides inclueded in the uppsala method?
Which step in the uppsala method ditermens withch fibers you have.
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