Carbohydrates / Polysaccharides - Structure elucidation - Chromatographic seperation of polysaccharides
6 important questions on Carbohydrates / Polysaccharides - Structure elucidation - Chromatographic seperation of polysaccharides
How can molecules differe form eachoter?
- Charge
- Size
- Conformation
- Polarity
What kind of Chrmatography methods are there?
- Anion-exchange
- Size Exclusion
- Adsorption
- Affinity
How does adsorption chromatography work?
So firs the linear polypeptides and later the branched.
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How does chromatography by size exclusion go?
Polysacharides DO NOT absorve UV light so detection is done by the refrective index in cobination iwth light scattering and or viscometric ditection.
How does anion exchange chromatography work?
The neutral ones will go out first, then the charged molecules.
By adding a salt or pH gradient you can let the charged molecules louse out of the colum. First the low charged ones will leave and later the more charged ones.
What can you say about the charge and the buffer?
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