Carbohydrates / Polysaccharides - Structure elucidation - Maldi Tof MS

4 important questions on Carbohydrates / Polysaccharides - Structure elucidation - Maldi Tof MS

How do you use MALDI-TOF MS?

First you ionise your sample. Then you measure your time of flight. The big ones will slower then small molecule. This flighing time relates to the mass of the compound.

What is the information you get form a MALDI TOF MS?

The degree of polymerization of the oligosaccharides as well as the substituent group (e.g. esters) can be recognized within minutes.

What are the positive things of the Maldi Tof MS?

  • Rapid technique
  • Quick impression on oligosacharides present
  • Esters are included in analysis.
  • Higher grades + faster learning
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What are the less pisitve things of the MALDI TOF MS?

  • No information with moleculair with less then 400 Dalton.
  • No distinction between different hexose, pentose because moleculair wight is the same.
  • Not really quantative

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