Carbohydrates / Polysaccharides - Structure elucidation - NMR

5 important questions on Carbohydrates / Polysaccharides - Structure elucidation - NMR

What are the two types of NMR used?

1H and 13C NMR

provides information on identification of the monosaccharides composition, elucidation of alfa and beta anomeric configuration and establishment of linkages pattern and sequences of the sugar units in oligo and polysaccharides.

How does 1H NMR work for carbohydrates?

It can determen if you have a Alfa or a Beta anomer.

How does 13C NMR work?

It can determine if you have a neutral or unronic acid.

More easy to see what C bound you have (alfa or beta) but less strong signal
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Why do you often have poor results with NMR?

  • High viscosity (in polysacharide solution), gives brad signal due to limited diffusion.
  • The neighboring interactions in a polymer can influance the signal.

How can you use NMR for analysis?

by hydrolysing it in olisacharides that you can diagnose.

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