Phenolic Compounds - Case 2: potato - Reaction with sulfite
20 important questions on Phenolic Compounds - Case 2: potato - Reaction with sulfite
What is the effect of vitamin C and sulfite?
Why is sulfite more efficient then vitamin C in preventing browning?
Sulfite inhibits the tyrosinase enzyme so the reaction can not occure anymore. Therefore you will need less.
Where does sulphite like to bind on the caffeic acid and why there?
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What does sulphite do when it is bound to the caffeic acid?
Why do the peaks lay more to the left in the socond graph?
The sulphite makes your compound more polair and therefore it will dilute faster.
Why could you have a loss of chloroginic acid?
- It could be that sulphite takes time to work and allready a part reacted with proteins
- At 320 NM the sulphite cloroginic acids have a different responce factor then the normal chloroginic acid molecules.
This is incase what happend and you have a hapochromic and bathochromic shift.
Calculations show that you have a quantative conversion.
What is the biggest error in canclualtion the molar extinction coefficient (E)?
What is the effect on the chloroginic acid by adding sulphite?
Why does red wine not become green?
How can you loose the red color?
Which are the 2 main components that can also react with phenolic compounds
- Cysteine
- Glutathione
They bought have a sulfide group.
Which is the stronger nucleophile: thiol, cystein or sulfite?
This is because the oxygen serounding the sulfide will draw some of the free electrons so therfore it is not as free as the electron pair in thiol and cystein.
cystein is stronger then glutathione because the Free electrons are presestn at the end of the molecule and not in the middle. The middle could mean that you have some steric hinder.
strongest: cystein, glutathione and sulfite.
With which machine can you determent where a sulpahte component will bind to in caffeic acid?
How can you see if there is couplin in H-NMR?
Hard to see for the small coupling and therfore you plot to an other measurements with slightly different settings against each other to get a 2D plot. In this you can clearly see if the signal is split up or not.
What ditermens if you have the sulfite group on the X, Z or Y position?
What is the inductive and resonance effect?
Resonance effect: double bonds are jumping around in the molecule.
Why is sulphite manly attached to the z-position?
Why is sulphite mainly attached to the y –position in chlorocatechol?
·Vicinal (partially) positive charges (+ and +) unfavourable
·Positive charge on xposition very unfavourable
·Position y and z seem equivalent, but y is the preferred site of addition (exp. evidence!)
·This might be explained by the stronger inductive electron withdrawing effect of chlorine compared to oxygen
Which position will the sulfite go to?
Where will the sulfite bind on the epicatechin?
- Position y is preferred, although charge distribution unfavourable
- Steric hindrance apparently overrules charge distribution
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