The three R's of DNA metabolism - Double standed break (DSB)

4 important questions on The three R's of DNA metabolism - Double standed break (DSB)

What kind of damage is repaired by DSB repair?

Damage due to ionizing radiation

What is the process of non-homologous end-joining (NHEJ)? (3 steps)

1. Damage recognized and DNA-PK and Ku bind
2. Blunt ends created
3. Ligation

What are two characteristics of non-homologous end joining (NHEJ)?

1. Major mode of DBS repair in higher eukaryotes
2. Usually deletes nucleotides at the break junction and is therefore mutagenic
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What are 2 characteristics of homologous recombination?

1. Most acvtive during S-phase
2. Used to rescue collapsed replication forks

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