Post transcriptional gene control III - Micro RNA (miRNA)
4 important questions on Post transcriptional gene control III - Micro RNA (miRNA)
What is the seed region of miRNA?
What are the 9 steps of biogenisis of miRNA and accociation with RISC?
2. Endoribonuclease DROSHA and associated protein bind pri-miRNA
3. DROSHA cleaves pri-miRNA strands
4. DROSHA dissociates releasing pre-miRNA (2 nucleotide overhang)
5. Pre-miRNA is transported to the cytoplasm by Exportin5
6. Dicer associated with TRBP binds pre-miRNA
7. Dices cleaves pre-miRNA strands (2 nucleotide overhang)
8. Dices dissociates releasing miRNA
9. Association with RISC of functional strand
What is the function of siRNA?
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How can you analyze miRNAs and their funciton?
2. Quantitative RT-qPCR
3. Microarray
4. Western blotting
5. Mass spectrometry
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
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