LE 2: Trancscriptional control of gene expression I - Prokaryotes

4 important questions on LE 2: Trancscriptional control of gene expression I - Prokaryotes

How is transcription initiated in prokaryotes?

Binding of sigma factor to promoter

How can DNA binding activity of activator and repressor proteins be regulated?

1. Expression
2. Conformational changes
3. Posttranslational modification

What three things does the interaction between the sigma factor and the promoter define?

1. Start point of transcriptional initiation

2. Direction of transcriptional initiation
3. Intensity of transcriptional intitiation
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So all sigma factor recognize the same promoters?

No, different sigma factors recognize different promoter sequences

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