LE 4: Transcriptoinal control of gene expression III - Chromatin

3 important questions on LE 4: Transcriptoinal control of gene expression III - Chromatin

What is the effect of modifications of the histone tail of the nucleosome?

Major change in chromatin state, acetylaton decreases interaction with DNA, trimethylation can lead to chromatin compaction

What is the process of heterochromatin formation at chromosome ends of S. Cerevisiae? (4 steps)

1. Rap1 recruits Sir3 and 4
2. Tehtering of Sir3 and 4 recruits Sir2
3. Sir2 deacetylates adjacent histone tails
4. Deacetylated histone tails recruit more Sir3 and 4 and contribute to spreading

What is the process of heterochromatin formation at chromosome ends of S. Pombe? (3 steps)

1. Suv39 trimethylates histone pairs
2. HP1 binds to the mehtyl groups
3. HP1 oligomerizes this leads to chromatin compaction

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