Post transcriptional gene control III - Ribosomal RNA synthesis

6 important questions on Post transcriptional gene control III - Ribosomal RNA synthesis

What is needed for pre-rRNA processing?

1. Complex
2. Endoribonucleases
3. Exoribonucleases
4. Modifying enzymes

How can you analyze ribosomal RNA and their precursors?

1. Northern blot hybridization
2. Quantitative RT-qPCR

What are the functions of snoRNPs?

1. Endonucleases
2. Ribosome methylation
3. Pseudouridinylation
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What is the funciton of  U3 snoRNPs in rRNA processing? (3 steps)

1. U3 snoRNP assembles into a large complex called the small subunit (SSU) processome
2. U3 snoRNP base-pairs with regions of the pre-rRNA
3. Base-pairing of other snoRNPs specify additional cleavage reactions that remove transcribed spacer regions

What are the three functions of methylation/pseudouridinylation?

1. Satbilization
2. More resistant to endocunleases
3. Catalytic process

What are the characterisitics of self-splicing introns of Goup I?

1. No branch point
2. Intorn conatains binding site for free G
3. Transeseterification
4. Linear product

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