Post transcriptional gene control III - Ribosomal RNA synthesis
6 important questions on Post transcriptional gene control III - Ribosomal RNA synthesis
What is needed for pre-rRNA processing?
2. Endoribonucleases
3. Exoribonucleases
4. Modifying enzymes
How can you analyze ribosomal RNA and their precursors?
2. Quantitative RT-qPCR
What are the functions of snoRNPs?
2. Ribosome methylation
3. Pseudouridinylation
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What is the funciton of U3 snoRNPs in rRNA processing? (3 steps)
2. U3 snoRNP base-pairs with regions of the pre-rRNA
3. Base-pairing of other snoRNPs specify additional cleavage reactions that remove transcribed spacer regions
What are the three functions of methylation/pseudouridinylation?
2. More resistant to endocunleases
3. Catalytic process
What are the characterisitics of self-splicing introns of Goup I?
2. Intorn conatains binding site for free G
3. Transeseterification
4. Linear product
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