LE 5: Post transcritptional gene control I - Splicing

4 important questions on LE 5: Post transcritptional gene control I - Splicing

What is the function of splicing factors?

The selection of splice sites by a base pairing interaction

What are the major splice factors?

U-rich small nuclear ribonucleoprotein particles (U snRNPs)

What are the 7 steps of splicing?

1. U1 and U2 snRNP bind splice site
2. U4, U5 and U6 snRNP form spliceosome
3. Intron will loop
4. Exons are closer to each other
5. U1 and U4 leave leading to rearrangement
6.  2 Transesterification reactions take place
7. Proteins degrade intron
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What are ATAC introns?

Special, low abundance snRNPs. They are minor spiceosomes and have AU at the 5'-end and AC at the 3'-end

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