Mapping eakaryote chromosomes by recombination - Mapping by recombinant frequency
8 important questions on Mapping eakaryote chromosomes by recombination - Mapping by recombinant frequency
What is the key to chromosome mapping?
Do, for any two specific linked genes, crossovers take place between them in all meiocytes
The closer/farther apart the genes are, the more likely that a crossover will take place and the higher/lower the proportion of recombinant products will be. Thus, the proportion of recombinants is a clue to the distance separating two gene loci on a chromosome map.
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Recombinant frequencies for different linked genes range from ... to ... percent, depending on their closeness. The farther apart genes are, the more closely their recombinant frequencies approach ... percent, and, in such cases, one cannot decide whether genes are linked or are on different chromosomes. What about recombinant frequencies greater than 50 percent? The answer is that such frequencies are never observed, as will be proved later
How many reciprocal recombinant products does a single crossover generate?
Genetic map unit (m.u.)
For example the recombinant frequency (RF) of 10.7 percent obtained by Morgan is defined as 10.7 m.u.A map unit is sometimes referred to as a centimorgan (cM).
Is the distance on a linkage map a physical distance along a chromosome?
Are the crossovers in adjacent chromosome regions independent events or does a crossover in one region affect the likelihood of there being a crossover in an adjacent region?
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