RNA: Transcription, processing, and decay - Transcription in eukaryotes
10 important questions on RNA: Transcription, processing, and decay - Transcription in eukaryotes
What is the function of transcription factors in gene transcription?
What is required to activate RNA polymerase II once it is positioned on the DNA as part of the pre-initiation complex?
Write down the order of events involving RNA polymerase II, leading to gene transcription.
- GTFs attract the core RNA polymerase II.
- Transcription begins after phosphorylation of the carboxy terminal domain (CTD) of RNA polymerase II.
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Is a fully transcribed RNA needed first before processes occur?
Do RNA processes occur in random order?
- Capping
- Splicing
- Cleavage and polyadenylation
Which protein is involved in all three RNA processes?
In which cellular compartment(s) do RNA processes take place?
Is alternative splicing an explanation for the observation that there are more proteins than genes in an organism?
Is splicing a tissue-specific event?
What do you expect the tissue-specific slicing may depend on?
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