Regulation of gene expression in eukaryotes - Lessons from yeast: the GAL system - Gal4 regulates multiple genes through upstream activation sequences

3 important questions on Regulation of gene expression in eukaryotes - Lessons from yeast: the GAL system - Gal4 regulates multiple genes through upstream activation sequences

In the presence of galactose, the expression levels of the GAL1, GAL2, GAL7, and GAL10 genes are 1000-fold or more higher than in its absence. True or false: in GAL4 mutants, however, they remain silent.

True

True or false. The genes GAL1, GAL2, GAL7 and GAL10 each have two or more Gal4-binding sites located at some distance 5′ (upstream) of its promoter

True

Because the Gal4-activated enhancers are located upstream (5′) of the genes they regulate, they are also called ....

upstream activation sequences (UAS)

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