Principle of fluorescence

4 important questions on Principle of fluorescence

When is there excitation in a fluotophore?

When light is absorbed with the energy that fits the energy difference between HOMO and LUMO

How does the fluorophore lose energy again after excitation?

Vibrational relexation

What is fluorescence emission?

Light is emitted when the electron jump back to the ground state
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Is there a difference between the absorbed and emitted wavelength?

Yes, the absorbed wavelength is slightly red shifted

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