Organelle specific probes

6 important questions on Organelle specific probes

What are 2 characteristics of membrane probes?

1. Hydrophobic
2. Anchored in the bilipid layer

What is a mitotracker?

Probe that partitions insto mitochondria independent of the membrane potential

How does potential and oxidation dependent dye work?

If there is small oxygen stress there is high fluorescence. If the organelle can handle Ros well there is low fluorescence
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How do probes for acidic compartments work?

The probe goes into the organelle, gets protonated and is trapped in the organelle

How do lysotrackers work?

The protonated group captures the probe into the lysosome

What are 2 characteristics of ion detecting probes?

1. Fluorophore changes characteristics in presence of calcium
2. Excitation when calcium is present

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