Fluorophore formation

3 important questions on Fluorophore formation

What type of formation is the formation of the fluorophore in GFP?

Posttranslational modification

What are the 4 steps of formation of the fluorophore of GFP?

1. Unfolded conformation
2. Cyclization
3. Dehydration
4. Oxidation

Can GFP be used in an anearobic environment?

No

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