Secretory pathway transport - vesicle based transport

6 important questions on Secretory pathway transport - vesicle based transport

What are the 9 steps of vesicle base transport?

1. Cargo selection
2. Budding
3. Scission
4. Uncoating
5. Transport
6. Thethering
7. Docking
8. Fusion
9. Disassembly

How are soluble proteins selected as cargo by coat proteins?

They are recognized by membrane cargo receptor proteins

What is retrograde transport?

Transport from golgi back to the ER. Makes use of COPI coat and KDEL protein
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What are the 3 steps of lysosomal targeting?

1. Posttranslational addition of mannose-6-phosphate
2. Mannose-6-phophate recognized by receptor
3. Included in vesicle to lysosome

How is a vesicle pinched off after budding?

Dynamin forms a ring around the neck and squeezes it of

How is a vesicle transported?

Kinesin 'walks' over microtubules

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