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1 Protein seperation
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1.1 Gel based
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What does 2D-PAGE stand for?
2 dimensional poly acrylamide gel electrophoresis -
What are the 2 dimensions you use for 2D-PAGE?
Charge
Size -
How do you divide the proteins by charge?
Iso electric focusing` -
How do you divide the proteins by size?
With the gel. It gets smaller to the end.
You treat the with SDS and heat them so they denature. The loading that they get form the SDS-PAGE will be equal to there size. -
What does SDS stand fore?
Sodium dodecyl substrate. -
How will the proteins move faster trought the PAGE?
Put a charge on the gel. -
How are proteins visualised?
By coomasie blue or silver strain. -
What does DIGE stand for?
Differential gel electrophoresis. -
What types of DIGE do you have?
With 2 (CY3, CY5) and with 3 DIGE (CY3, CY5, CY2) -
1.2 HPLC
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What does HPLC stand for?
High pressure liquid chromatography.
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