Protein seperation - HPLC
9 important questions on Protein seperation - HPLC
Why is there pressure in the HPLC?
How does a column based on size work.
How does a column based on size work.
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How does a column based on Affinity work?
What are the aplications of HPLC?
Identification
Purificaton
Quantification
How does the detector detect a protein?
Or by ultraviolet absorbiton
fluorescence (if the proteins are labeld)
Redioactivity (if the protiens are labled)
Electrochemistry: if the portiens underwent an oxidation or reduction reaction)
What does MuDPIT stand for?
How do the Colums let go of the proteins?
What is the benefit of MuDPIT?
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