Analyzing which proteins - MS-TOF - Ionisation

6 important questions on Analyzing which proteins - MS-TOF - Ionisation

How does MALDI work?

A laser gives the peptides energy.

What does SELDI stand for?

surface enhanced laser desorption/ionisation

How does SELDI work?

Same as MALDI only now you ar not analysing all the proteins just looking if 1, 2 or 3 proteins are present. This makes it a very good analyser for body fluids.
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What does ES stand for?

Electron spray ionization

How does ES work?

Liquid sample is used and put in to small droplets sprayed with ions and slowly the liquid is evaporated and you only have the ionized peptide left.

What are the nice tings about ES?

You can immediately use it after HPLC, no more further treatment is needed.

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